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Introduction to Bioinformatics Microarrays1: Microarray Technology Course 341 Department of Computing Imperial College, London Moustafa Ghanem Aims for the 2nd part ...
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Review of microarray technology from data/database perspective ... 'An observed phenotype is specific for ... Rosetta Resolver (www.rosettabio.com) OpenSource ...
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... available for download from Web to run on your computer 3. Integrated with NCI ... K-means clustering finds K clusters of similar genes. ...
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Each array location is typically known as a probe and contains ... (Rspot, Rbkg), (Gspot, Gbkg) Data Mining. Classification. Statistical Analysis. Motivation ...
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Online documentation (HTML manual, tutorials, examples, etc.) on Web site ... Reports: dynamic genomic Web-accessible spreadsheets, tab-delimited data for Excel ...
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Semester project: Microarrays and Statistics Part 2 of 2: Introduction to Microarrays
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... threats, including bioterrorism increases the burden ... Bioterror Viruses. POX: Pox viruses. VAC: Vaccinia. VAR: Variola (Smallpox) MPV: Monkeypox Viruses ...
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... database for the silkworm microarray data. (http://silkworm.swu.edu.cn/ microarray ... All raw data and tissue-specific genes were viewed in this database. ...
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Removal of outliers. Absolute measurements. cDNA microarray ... Can we make better treatment decisions for a cancer patient based on gene expression profile? ...
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DNA in somatic cells, all cells except the gametes, is the same ... disease-free based on both clinical and radiological testing. ... (digital camera) ...
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A Quantitative Overview to Gene Expression Profiling in ... Pie-Bald black. Non-Pie-Bald black. Normal. White. Recessive. SA-1 = 53 = 125. N of Configurations? ...
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The conentration of labels in the cDNA is measured! ... gar. Gene 6. 0.86. 0.84. 0.42. 0.84. 0.44. groo. Gene 5. 0.41. 0.21. 0.11. 0.26. 0.60. bas. Gene 4 ...
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Microarray-DNA microarray hybridization is a method of choice for high-throughput RNA expression analysis.-It s a high throughput technology.
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Microarray Basics Part 2: Data normalization, data filtering, measuring variability Log transformation of data MA plots Non linear normalization Spatial defects over ...
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cDNA Microarray Design and Pre-processing By H. Bj rn Nielsen Why Experimental Design To enable statistical hypothesis verification/falsification To balance the ...
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Protein microarrays Martyna Franczuk Functional protein microarrays differ from analytical arrays in that functional protein arrays are made of full-length functional ...
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Allows study of thousands of genes at same time. Glass of ... Fabrications of Microarrays. Size of a microscope . Images: http://www.affymetrix.com ...
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MICROARRAY DATA REPRESENTED by a N M matrix contains the gene expressions for the N genes of the jth tissue sample (j = 1, ,M). N = No. of genes (103 - 104)
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These antibodies can be made in large quantities and have a specific affinity ... the Methodist Hospital Houston, TX, USA, prior to commencement of radiotherapy. ...
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'Spot' them on a medium, e.g. an ordinary glass microscope ... Spot localization and detection, including the extraction of the background ...
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Microarray Pitfalls Stem Cell Network Microarray Course, Unit 3 October 2006 Goals To provide some guidelines on Affymetrix microarrays: How to use them How not to ...
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DNA Microarray: A Recombinant DNA Method Basic Steps to Microarray: Obtain cells with genes that are needed for analysis. Isolate the mRNA using extraction buffer.
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Microarray Basics Part 1: Choosing a platform, setting up, data preprocessing Experimental design What type of microarray What overall design strategy How many ...
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Suppression Subtractive Hybridization (SSH) Differential screening (plus/minus ... Suppression Subtractive Hybridization (SSH) technique isolates low abundant ...
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Jamie Mashek What we will be discussing What is DNA microarray? The purpose of using DNA microarray. The plate. Steps to perform a microarray.
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Experimental Gerontology 40: 737-744 (2005) ...
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'A microarray is a tool for analyzing gene expression that consists of a small ... Pyxis CattleArray 7600. Bovine total leukocyte cDNA library & immune gene amplicons ...
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Microarrays are simply small glass or silicon s upon the surface of which ... Use thousand of oligos that 'tile' or span the genomic DNA for characterization. ...
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Microarray analysis as a prognostic and predictive tool: are we ready? Enzo Medico Laboratory of Functional Oncogenomics Institute for Cancer Research and Treatment
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DNA Microarrays O que DNA Microarray? Tamb m conhecido como chip de DNA Permite a medida do n vel de transcri o de cada gene no genoma (express o dos genes ...
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13 week-old C57BL/6 mouse (Ovariectomy 11week-old) Soy-free diet for 1 week. 100 mg /kg x day ... Harvest. organ. Total RNA. Isolation. Affymetrix GeneChip ...
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Microarray Data Set The microarray data set we are dealing with is represented as a 2d numerical array. Characteristics of Microarray Data High dimensionality of gene ...
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Clustering microarray data 09/26/07 Overview Clustering is an unsupervised learning clustering is used to build groups of genes with related expression patterns.
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Title: MicroArray Image Analysis Author: Robin Liechti Last modified by: Carol Bult Created Date: 2/22/2002 1:15:29 PM Document presentation format
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Title: Microarray Data Analysis Author: Kath Last modified by: Janet Murray Created Date: 10/14/2003 5:52:40 PM Document presentation format: On-screen Show
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Protein Lysate Microarrays Clay Scott Ryan McConnell Shannon Neeley
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V7 Genexpression - Microarrays Idee: analysiere Ko-Expression von mehreren Genen um auf funktionelle hnlichkeiten zu schlie en wichtige Fragen:
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Databases for Microarrays Vidhya Jagannathan SIB, Lausanne Overview Microarray data in a nutshell Why databases? What data to represent? What is a database?
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Microarray Image Processing
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Microarray Data Analysis March 2004 Differential Gene Expression Analysis The Experiment Micro-array experiment measures gene expression in Rats (5000 genes).
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Title: Slide 1 Author: Letizia Magnoni Last modified by: Simone Created Date: 3/9/2004 3:06:32 PM Document presentation format: Presentazione su schermo
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Epistasis Analysis Using Microarrays Chris Workman
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Printing High Quality Microarrays
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Lysis: The dissolution or destruction of cells by the action of a specific lysin ... cellular debris and fluid produced by lysis. Protein Lysate Array Design ...
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BASE and storage of microarray data (Johan van Heerden) ... Normalization (Excel, DNMAD, MIDAS) (Shane Murray) linear regression ... best imputation method ...
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L15:Microarray analysis (Classification)
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Basics on microarrays. Concepts of cDNA, mRNA, dyes, probes, hybridization, ... How to resolve the errors and bias introduced by the microarray experiment ...
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Probe selection for Microarrays Considerations and pitfalls
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... (hybridize), and labeled DNA will be detected on photographic film ... Correct bias in MA plot for each print-tip. Correct bias in MA plot for each sector ...
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Title: Analysis of Affymetrix Microarrays Author: Gareth Palidwor Last modified by: gpalidwor Created Date: 1/18/2005 5:20:25 PM Document presentation format
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Gene, MicroArray and GAs Ashish Anand Kanpur Genetic Algorithms Laboratory (KanGAL) IIT Kanpur Overview What are Genes ? Why study Genes ? What is MicroArray ?
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The Central Dogma of ... Reverse the hybridization - put the probes on the filter and ... Bolstad, B.M., Irizarry R. A., Astrand, M., and Speed, T.P. (2003) ...
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Identify genes with differential gene expression between two ... Triangle rule dik = dij djk. Euclidean distance d = S ( xi yi) 2 over i = 1 to n. ...
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Introduction to Affymetrix Microarrays Why use microarrays? Permits expression profiling of thousands of genes in parallel Why use Affymetrix microarrays?
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DNA chip drawbacks: Possibly provide too much data ... DNA microarray drawbacks: Do not measure as great a variety of hybridization ...
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