Biological Phenotype of HIV1 BG recombinants and other genetic forms from Galicia, Spain.

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Biological Phenotype of HIV-1 BG recombinants and
other genetic forms from Galicia, Spain.

• L Pérez-Alvarez1, M Muñoz-Nieto1, E Delgado1,
  G Casado1, M Sierra1, R Carmona1, M Thomson1,
  S Pérez2, Y Vega1, C Miralles2, E Vázquez de
  Parga1, A Ocampo2, G Contreras1, L Medrano1,
  JA Taboada3 and R Nájera1.

1 Viral Pathogenesis Department. CNM. Instituto
de Salud Carlos III, Majadahonda, Madrid, Spain
2 H. Xeral Cíes de Vigo, Pontevedra, Galicia,
Spain 3 Consellería de Sanidade e Servicios
Sociais. Xunta de Galicia, Spain
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BIOLOGICAL CHARACTERIZATION OF HIV-1
SYNCYTIUM INDUCING (SI), CELLULAR TROPISM AND
CORECEPTOR USAGE
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Bioinformatics methods for predicting coreceptor
usage
X4 SI T-lymphotropic
R5X4 SI dual-tropic
R5 NSI Macrophage-tropic
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OBJECTIVES

• To investigate the biological characteristics of
  different genetic forms, emphasizing the BG
  recombinants most frequently detected in Galicia,
  Spain.

• To define the association of these properties
  with V3 loop sequence.

• To investigate the correlation between
  biological characteristics of RF and the
  evolution of the infection.

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31 Patients Non-B subtypes and RFs
COUNTRY OF ORIGIN
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NON-B SUBTYPES HIV-1 PRIMARY ISOLATES 50 samples
from 31 patients Distribution according to V3
Subtype
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RFs 36/50(72)
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SYNCYTIUM PHENOTYPE ACCORDING TO HIV-1 V3
SEQUENCE
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Biological characteristics of HIV-1 primary
isolates of different genetic forms during the
evolution of the infection
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CONCLUSIONS

• A high frequency (72) of Non-B HIV-1 Primary
  Isolates were RFs representative of the RFs
  identified in Galicia
• The Biological Phenotype of Non-B Subtypes and
  Recombinants is linked to V3 Loop sequence, being
  this correlation similar to that has been
  described for subtype B strains.
• Predicted Phenotype defined by Geno2pheno
  bioinformatic program was in relation with
  Biological Phenotype.

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CONCLUSIONS

• Characteristic mutations at V3 loop of CRF14_BG
  primary isolates have been described
• Different substitutions and deletions, including
  atypical changes at GPG motif of some URFs were
  detected

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CONCLUSIONS

• The Biological Phenotype of all BG Recombinants
  (CRF14_BG and URFs) was SI/X4, independently of
  the years of evolution of the infection.
• The high frequency of SI/X4 phenotype among RFs
  primary isolates,even at early stage of the
  infection, emphasize the need for studying the
  disease progression in these patients.

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