Aucun titre de diapositive

1
(No Transcript)
2
Somite Maturation
Epithelial immature
SOMITE
dm
my
Differentiating/maturing
sc
3
Mouse Ptprq/Mrf4/Myf5 locus
YAC 5' -200
Sal I -90
Cla I -60
Spe I -34
Ptprq
Mrf4
Myf5
-150
Other genes

• Mrf4 ko mice
• 3 alleles -gt all perturb Myf5 expression
• all 3 display distal rib phenotypes

4
Neomycin
Neo origins 1. pMC1Neo (Stratagene)
mutant 2. pNHBC (pMC1Neo Stratagene, M.
Cohen-Tannoudji/F. Jassier) non-mutant 3.
RNApolII-Neo-HPRTpA (M. Capecchi) mutant 4.
PGK-Neo-bpA (P. Soriano) mutant 5. PGK-Neo-
pgkpA(pPNT) mutant
see Yenofsky et al. (1990). PNAS 87 3435. G -gt
T conversion at pos. 2096 (loss of 246 bp XhoII
fragment) aa182 glutamate GAG -gt aspartic acid
GAT
see Mortensen et al. (1992). Mol. Cell. Biol.
12 2391. High G418 doses to generate homozygous
ES KO cells. PGK-Neo (origin?)
Neo effects in cis (N.B. cryptic splice site in
Neomycin) 1. Pham et al (1996). PNAS
9313090 Granzyme B and b-globin loci 2. Olson
et al. (1996). Cell 85 1 Mrf4 ko 3. Moran et
al. (1999). Nature 399742. Radical fringe ki