Extraction of DNA

1
Extraction of DNA

• Lab 9

2
Step 1 Extract DNA from Banana

• Follow the instructions listed on your handout.
• (Steps 1-9)
• Materials on the cart Amt you need
• -Shampoo 1 teaspoon
• -Salt 2 pinches
• -Vial w/chilled 91 alcohol 1 vial/pair
• -De-ionized water 20 ml
• -Banana mixture 3 teaspoons
• Important notes Reminders
• -Note the appearance describe what you see.
• -Rinse out your vials after you have made your
  observations
• -Answer questions 1-4

3
Step 2 Extracting Your DNAhttp//www.pbs.org/wgb
h/nova/teachers/activities/2809_genome.html

• Supplies are in the back of the room.
• 1) Pick up a dixie cup w/ salt water (2 tsp)
• 2) Swish around in your mouth thoroughly for 30
  sec.
• 3) Spit back into the cup
• 4) Pour the contents of the cup into the vial
  with soap solution.
• 5) Gently rock the vial back forth (3 min)
• 6) Take one pipette full of chilled alcohol and
  slowly add it to the vial while tilted making
  sure that they dont mix.
• 7) Let stand for 1 minute
• 8) Gently mix the alcohol into the soap layer
  with a glass rod.
• 9) DNA should precipitate (whitish) Be patient

4
Final Steps

• 10) Collect your DNA using a pipette or stirring
  rod and place into the capped containers. Add
  alcohol to preserve it.
• 11) Seal and secure with glue.
• 12) Write your initials on container (very small)
• Place your DNA in the container w/your period
• Pick up your DNA at lunch
• 15) Clean up Rinse out the vials
• Throw out paper cups
• Please refill your vial with one pipette full
  of soap and place it back in the rack before you
  leave.
• Thanks have a Great Thanksgiving!!!

5
Summary of Results -Due Friday Dec. 2

• Title
• Introduction/Background
• Separation of DNA Explain the process
• -Why does this technique work?
• -Why do you need salt, shampoo, alcohol?
• Results Explain what you observed
• Describe what you saw
• How was the Banana DNA different from your DNA?
• How could you verify that what you have is DNA?
• Conclusion/Evaluation
• How could this lab activity be improved? (Sources
  of error)
• What else could be used? Other techniques?

6
Upcoming Labs

• Lab 10 Bacterial Transformation (done in
  class)
• Lab 11 Analysis of DNA w/ Gel Electrophoresis
• Both are AP recommended labs and must be done.
• Important note Lab 11 will require extra
  time to do.
• -This lab will be done after school on TWO days
• -You will choose which day you can come. (sign
  up list)
• -The time needed to do the lab 2 hrs
• -Please let your parents know about this
  upcoming lab
• Possible Days to choose from
• 1st Day Mon. December 5th 330pm to 530pm
• 2nd Day Wed. December 7th 330pm to 530pm