Fig' 8'2

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Fig. 8.2
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Genomic DNA
BamHI
BamHI
BamHI
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Commonly Used Vectors

• Vector Cloning Capacity
• Plasmid lt 15 kb
• Bacteriophage Lambda lt 25 kb
• Cosmid 30-45 kb
• Bacteriophage P1 70-90 kb
• Bacterial Artificial Chromosome 100-500 kb
• Yeast Artificial Chromosome 250-1000 kb

From Table 8.2
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1. EcoRI Digestions
2. Ligate
3. Transform
4. Select
Fig. 8.5
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Genomic Libraries

• Vector clones in genomic equivalent
• Plasmid 200,000
• Lambda Phage 150,000
• P1 Phage 38,000
• YAC 6,000

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Isolate RNA
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Fig. 8.19
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Fig. 8.19
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Genomic Southern Blot Genotype m/m
WT
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