GLYCOBASE

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GLYCOBASE ustl.univ-lille1.fr/glycobase/
Constitution of a database of O-glycan
standards E. Maes, K. Thackray, Y. Guérardel and
G. Strecker Unité de Glycobiologie Structurale
et Fonctionnelle, UMR 8576, Villeneuve dAscq,
FRANCE
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Study of glycoconjugate biodiversity

• Main models
• Amphibians
• Invertebrates
• Funga
• Bacteria

• Glycoconjugates
• N-glycans, O-glycans
• Free oligosaccharides
• Polysaccharides
• Lipopolysaccharides

O-glycans

• More than 400 new glycan structures described
• Many of them result from unknown activities

A. tigrinum
C. elegans
X. laevis
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Aim of glycobase
Put together all the O-glycans structures we
have described to make them available for
glycobiologists. Standards for structural work
Acceptor substrates for glycosyltransferases
Example
Study of a divergent (a1-4)Fucosyltransferas
e in Xenopus tropicalis Dr A. Maftah, Equipe de
Glycobiologie et Biotechnologie
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Present and future work

• Expend the base to all the species we have
  studied
• In collaboration with Dr K.H. Khoo (Academia
  Sinica, Taiwan), systematic CID-MS/MS
  fragmentation of our O-glycan collection
• Include NMR database we are currently developing
• Based on the used of easily identified signals
• a and b anomers
• Fucose CH3
• Acetyl signals

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CREDITS

• Emmanuel MAES NMR engineer
• Kevin THACKRAY Student