Lab 5: Cellular Respiration

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Lab 5 Cellular Respiration

• Description
• using respirometer to measure rate of O2
  production by pea seeds
• non-germinating peas
• germinating peas
• effect of temperature
• control for changes in pressure temperature in
  room

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Lab 5 Cellular Respiration

• Concepts
• respiration
• experimental design
• control vs. experimental
• function of KOH
• function of vial with only glass beads

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Lab 5 Cellular Respiration

• Go to the following lab bench simulation1.)
  Identify the raw materials and products of the
  process of cell respiration.
• 2.) Name three processes in plants which
  require ATP.
• 3.) List three ways the rate of cell
  respiration can be measured.

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• 4.) What does a respirometer measure?
• 5-6.) List five key features of a respirometer
  and discuss what each feature does. Draw a
  respirometer, labeling these key features.
• 7.) Explain how a respirometer functions.
  Include the role of K2CO3 in your discussion.

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Lab 5 Cellular Respiration
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• 8-9. Write a complete procedure for this lab
  activity and use drawing to assist your
  description of the procedure.

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• 10.) What is the reading of the pipette in the
  how to read a pipette page of the lab bench
  activity on respiration?
• 11.) Describe the non-metabolizing control in
  the respiration experiment simulation. Explain
  specifically why this control is needed.

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• 12.) It is important to know that nongerminating
  seeds are not dead they are dormant. Do they
  respire? Why?
• 13.) Thought question (why must this lab be
  carried out on peas before they form leaves and
  explain how the formation of leaves would cause
  the results of this lab to be in error)

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• 14.) Gas volume may change due to temperature
  changes in a respiration investigation. In such
  an investigation, the experimenter must correct
  for differences in volume that are due to
  temperature fluctuation rather than rate of
  respiration. Explain how this is done.

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Analysis of Results I

• 15.) On the Analysis of Results I web page
• calculate the rate of oxygen consumption with
  respirometer pipette pictures given.Show the
  appropriate formula and all work.

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• 16.) Complete the four lab quiz questions,
  writing out the letter of the answer along with
  the complete foil of the answer.

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Lab 5 Cellular Respiration

• Conclusions
• ?temp ?respiration
• ?germination ?respiration

17-18. Calculate the rate of oxygen consumption
for the time interval from 0 to 20 minutes for
the four lines on the graph.Show your work.19.
Suggest a correct title for this graph
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Lab 5 Cellular Respiration (review and research)

• 19-25. The results below are measurements of
  cumulative oxygen consumption by germinating and
  dry seeds. Gas volume measurements were corrected
  for changes in temperature and pressure.
• 19. Plot the results for the germinating seeds
  at 22C and 10C.
• Calculate the rate of oxygen consumption for the
  germinating seeds at 22C, using the time
  interval between 10 and 20 minutes.
• 21- 22. Account for the differences in oxygen
  consumption observed between
• -- germinating seeds at 22C and at 10C
• -- germinating seeds and dry seeds.
• Define the term Q-10
• Define the terms poikilothermic and homeothermic