Fig. S3.

1
Fig. S3.
Fig. S3. Detection of sugars released from RS by
R. bromii using thin layer chromatography (TLC).
Cultures were incubated for 48h in YCFAS and M2S
medium containing 0.2 RS2 (HM-958) or RS3
(NL-330). Samples are shown pre-incubation (t0)
and after 48 hour incubation (t48). Glucose (glu)
and maltose (mal) are shown as standards. Thin
layer chromatography- 10 mg/ml solutions of
glucose, maltose, isomaltose, panose,
maltotriose, maltotetraose, maltopentaose and
pullulan were prepared in basal YCFA or M2 medium
for use as standards. Samples from R. bromii
cultures were centrifuged (13000g, 10min), and
800 µl of the supernatant was mixed with 200 µl
of acetic acid before being vacuum concentrated
to 20 µl. The concentrate was resuspended in 30
µl of 50 ethanol standards were treated in the
same way. 1 µl of concentrate was applied to
Silica gel TLC plates (PartisilK5, 250 µm) and
sugars separated using isopropanol, ethyl acetate
and H2O solvent in a ratio of 13189. Spots were
visualized by spraying the plate with 0.2
orcinol in sulphuric acid methanol (10 90) and
heating at 105ºC (approx. 10 min.).