Exercise 1

1
Exercise 1

• Microscopy
• Begin Environmental Isolate

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MICROSCOPY Types Part 1

• Bright-field
• The most common form of light microscopy.
  Extensively used for the visualization of
  microorganisms usually necessary to stain
  specimens for viewing.

Enterococcus
Anthrax spore stain
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MICROSCOPY Different Types

• Dark-field Used for viewing live, unstained
  material. The specimen appears bright on a dark
  background. For low- or medium-power work.

Diatom
Rotifer
Cyanobacteria Nostoc
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MICROSCOPY Types
Amoeba

• Phase contrast Microscopy used when a colorless
  specimen, which absorbs little light, (e.g. a
  non-pigmented living cell) is not clearly visible
  by bright-field microscopy

Bacteria
Rotifer
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MICROSCOPY Types

• Fluorescence microscopy
• Fluorochrome treated specimens (fluorescent
  stained) are irradiated with ultra-violet
  radiation and the light emitted forms the image
  of the specimen in a manner similar in principal
  to that in bright-field microscopy. The
  fluorescent scope is designed so that the
  specimen can be illuminated at one wavelength of
  light and observed by light emitted at a
  different wavelength.

Yersinnia pestis
Rhizopus rot-Black bread mold