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Department of Anatomy and

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Alizarin red stained preparations. 5 mm. Discrete mineralisation ... Alizarin red stain for calcium. 2 mm. Dystrophic mineralisation of osteoblast cell layers ... – PowerPoint PPT presentation

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Title: Department of Anatomy and


1
Tim Arnett
Department of Anatomy and Developmental
Biology University College London
2
Trabecular bone nodules formed by primary
osteoblasts
Unstained preparations
3
Trabecular bone nodules formed by primary
osteoblasts
Unstained preparations
4
Acidosis inhibits mineralised bone nodule
formation by rat osteoblasts
pH 7.4
pH 6.9
Alizarin red stained preparations
5
Discrete mineralisation of trabecular bone
matrix formed by primary osteoblasts cultured
with 2 mM ?-glycerophosphate
Alizarin red stain for calcium
6
  • Dystrophic mineralisation of osteoblast cell
    layers
  • ? cell death inhibition of normal bone matrix
    formation
  • in presence of 10 mM ?-glycerophosphate

7
Hypoxia inhibits proliferation and bone formation
by osteoblasts
8
Alkaline phosphatase staining and
matrix mineralisation in primary osteoblast
cultures
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