Department of Anatomy and

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Tim Arnett
Department of Anatomy and Developmental
Biology University College London
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Trabecular bone nodules formed by primary
osteoblasts
Unstained preparations
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Trabecular bone nodules formed by primary
osteoblasts
Unstained preparations
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Acidosis inhibits mineralised bone nodule
formation by rat osteoblasts
pH 7.4
pH 6.9
Alizarin red stained preparations
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Discrete mineralisation of trabecular bone
matrix formed by primary osteoblasts cultured
with 2 mM ?-glycerophosphate
Alizarin red stain for calcium
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• Dystrophic mineralisation of osteoblast cell
  layers
• ? cell death inhibition of normal bone matrix
  formation
• in presence of 10 mM ?-glycerophosphate

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Hypoxia inhibits proliferation and bone formation
by osteoblasts
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Alkaline phosphatase staining and
matrix mineralisation in primary osteoblast
cultures