Title: Department of Anatomy and
1Tim Arnett
Department of Anatomy and Developmental
Biology University College London
2Trabecular bone nodules formed by primary
osteoblasts
Unstained preparations
3Trabecular bone nodules formed by primary
osteoblasts
Unstained preparations
4Acidosis inhibits mineralised bone nodule
formation by rat osteoblasts
pH 7.4
pH 6.9
Alizarin red stained preparations
5Discrete mineralisation of trabecular bone
matrix formed by primary osteoblasts cultured
with 2 mM ?-glycerophosphate
Alizarin red stain for calcium
6- Dystrophic mineralisation of osteoblast cell
layers - ? cell death inhibition of normal bone matrix
formation - in presence of 10 mM ?-glycerophosphate
7Hypoxia inhibits proliferation and bone formation
by osteoblasts
8Alkaline phosphatase staining and
matrix mineralisation in primary osteoblast
cultures