Diapositiva 1

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Tissue Microarray Platform Galileo TMA CK3500
Manuela Maffè
Workshop on Tissue Microarrays in Translational
Research October 18th, 2006 BioRep S.r.l., Milan
(Italy)
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Objective
Development of a high throughput Tissue
Microarray platform for a streamlined process
from the tissue collection to the single spot
analysis, passing through TMA construction
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The existing Tissue Microarrayers
Beecher Instruments, USA
Alphelys, France
Beecher Instruments, USA
Chemicon International, USA
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Galileo a new tissue arrayer concept
Construction
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Galileo a new tissue arrayer concept
Construction
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Galileo a new tissue arrayer concept
Construction
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Galileo a new tissue arrayer concept
Construction
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Galileo a new tissue arrayer concept
Construction
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IseTMA software
Template Edit
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IseTMA software
TMA Design
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IseTMA software
TMA Construction

• Identify the punch areas on the donor blocks

• Load the design file

• Insert the paraffin blocks

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IseTMA software

• Overlap the reference slide to the donor block
• Align the images looking into the monitor
• Save the punch positions

I. Manual Overlapping
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IseTMA software
II. Digital Overlapping
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IseTMA software
TMA Construction

• Identify the punch areas on the donor blocks

• Load the design file

• Insert the paraffin blocks

• Sample

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IseTMA software
Excel Data Review

• Personalize the report

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IseTMA software

• Associate the clinico-pathological data to the
  corresponding donor blocks
• Add the results of the analyses
• Insert images

Excel Data Review
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The Tissue Microarray Platform
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The Tissue Microarray Platform
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Acknowledgements
Integrated Systems Engineering Team

• Pasquale De Blasio, President CEO
• Maurizio Falavigna, Research Development
  Manager
• Manuela Maffè, Research Development Engineer

Thanks to

• Stephen M. Hewitt - TARP Lab, NCI, NIH, Bethesda,
  MD, USA
• Anna Sapino Anatomia Patologica Unità di
  Senologia, Az. Osp. Molinette, Torino, Italy
• Massimo Barberis MultiLab, Osp. Multimedica,
  Milan, Italy
• Ida Biunno ITB-CNR, Milan, Italy

Project financed by the

• Ministero Attività Produttive MAP Regione
  Lombardia

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TMARep Tissue MicroArray Database
TMARep is a web-based tissue bank tissue
microarray oriented

• It represents the data storage to support tissue
  array creation and analysis.
• The site provides
• the possibility of inserting patient clinical
  data associated to samples (age, gender, year of
  birth, race, familiarity for this or other
  pathologies...)
• the possibility of inserting sample description
  data (type of surgical operation, topography,
  temporary diagnosis)
• a RDBMS to store own proper TMA experiment data
• the possibility of searching through all shared
  information uploaded into the database, in order
  of data, tissues and image sharing
• an images warehouse to allow a virtual pathology
  development.

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TMARep Tissue MicroArray Database
Graphical User Interface clinical sample data
submission
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TMARep Tissue MicroArray Database
Users management
complete overview possibility of modifying
everything about his/her data.
Supervisor
short data form overview of data belonging to
his/her institution only possibility of
inserting and modifying new samples.
Pathologist/ technician
overview of all data into the database no
possibility of handling any kind of data.
Researcher
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TMARep Tissue MicroArray Database
Future authomatic Biorep e-mail query
Graphical User Interface selection sample data
summary
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TMARep Tissue MicroArray Database
Image uploading

• After microbiological and informatics
  analisys, tissue images can be uploaded.
• Also comments, diagnosys and analisys results
  can be upload with them.
• These new information are visualized in the
  page of sample description, after previous data.