PRACTICAS DE LABORATORIO EN REPRODUCCION

1
PRACTICAS DE LABORATORIO EN REPRODUCCION

• INVESTIGACION EN REPRODUCCION

2
HISTOLOGIA

• Gonzales GF, Hartinger S, Flores Y, Zárate K, Yi
  P. Cycloheximide prevents production of
  arresting, a fraction of 30-50 kDa obtained from
  seminiferous tubules conditioned medium An
  evidence that this fraction is produced in vitro.
  Asian Journal of Andrology. 2004 6 359-364.
• Gonzales GF, Miranda S, Nieto J, Fernandez G,
  Yucra S, Rubio J, Yi P, Gasco M. Red Maca
  (Lepidium meyenii) reduced prostate size in rats.
  Reproductive Biology and Endocrinology. 2005
  3(1)5

3
Izquierda ControlDerecha Arresting
4
Control Paracrino

• Hedger MP, Khatab S, Gonzales GF, de Kretser DM.
  Acute and short term actions of serotonin
  administration on the pituitary-testicular axis
  in the adult rat. Reproduction, Fertility and
  Development. 19957 1101-1109.

5
ESPERMATOGENESIS

• Gonzales GF, Gasco M, Córdova A, Chung A, Rubio
  J, Villegas L. Effect of Lepidium meyenii (Maca)
  on spermatogenesis in male rats acutely exposed
  to high altitude (4340 m). Journal of
  Endocrinology 2004, 18087-95

6
TRANSILUMINACION

• Gonzales GF, Del Valle L. Adult rat seminiferous
  tubules secrete a fraction greater 30 KDa to
  regulate spermatogenesis. Human Reproduction
  199510 1435-1443.
• Almenara A., Escalante G., Gazzo E., Gonzales GF.
  Transillumination to evaluate spermatogenesis
  Effect of testosterone enanthate in adult male
  rats. Archives of Andrology. 2001 4621-27.
• Gonzales GF, Ruiz A, Gonzales C, Villegas L,
  Córdova A. Effect of Lepidium meyenii (Maca)
  roots, a Peruvian plant on spermatogenesis of
  male rats. Asian Journal of Andrology. 2001
  3231-233.
• Gonzales GF, Hartinger S, Flores Y, Zárate K, Yi
  P. Cycloheximide prevents production of
  arresting, a fraction of 30-50 kDa obtained from
  seminiferous tubules conditioned medium An
  evidence that this fraction is produced in vitro.
  Asian Journal of Andrology. 2004 6 359-364.
• Chung F, Rubio J, Gonzales C, Gasco M, Gonzales
  GF. Dose-response effects of Lepidium meyenii
  (Maca) aqueous extract on testicular function and
  weight of different organs in adult rats. Journal
  of Ethnopharmacology. 2005 98143-147.

7
CONTEO DE ESPERMATIDES EN TESTICULO

• Gonzales C, Rubio J, Gasco M, Nieto J, Yucra S,
  Gonzales GF. Effect of short-term and long-term
  treatments with three ecotypes of Lepidium
  meyenii (MACA) on spermatogenesis in rats.
  Journal of Ethnopharmacology. 2005. En prensa.

8
CONTEO Y MOVILIDAD DE ESPERMATOZOIDES EN EPIDIDIMO

• Del Valle LJ, Bustos-Obregón E, Gonzales GF.
  Serum reproductive hormone levels and sperm
  production in male adult rats after treatment
  with arresting, a fraction obtained from
  seminiferous tubules conditioned medium.
  Andrologia 2003 35 351-357.
• Gasco M, Rubio J, Chung A, Villegas L, Gonzales
  GF. Effect of high altitude exposure on
  spermatogenesis and epididymal sperm count in
  male rats. Andrologia 2003, 35 368-374.
• Gonzales GF, Rubio J, Chung A, Gasco M, Villegas
  L. Effect of alcoholic extract of Lepidium
  meyenii (Maca) on testicular function of male
  rats. Asian Journal of Andrology 2003 5 349-352
• Gonzales GF, Gasco M, Córdova A, Chung A, Rubio
  J, Villegas L. Effect of Lepidium meyenii (Maca)
  on spermatogenesis in male rats acutely exposed
  to high altitude (4340 m). Journal of
  Endocrinology 2004, 18087-95

9
Epididymal sperm count (106) in male rats treated
for 7 and 42 days with vehicle, Red Maca, Yellow
Maca or Black Maca. Plt0.05 with respect to the
control group.
10
APOPTOSIS DE CELULAS GERMINALES
11
CONTAMINANTES EN REPRODUCCION

• Eibensteiner L, Del Carpio-Sanz A, Frumkin H,
  Gonzales C, Gonzales GF. Lead exposure and semen
  quality among traffic Police in Arequipa, Peru.
  International Journal of Occupational and
  Environmental Health 2005, 11 161-166.
• Bustos-Obregon E, Yucra S, Gonzales GF. Lepidium
  meyenii (Maca) reduces spermatogenic damage
  induced by a single dose of malathion in mice.
  Asian Journal of Andrology. 2005 771-76.

12
INTERCAMBIO DE CROMATIDES HERMANAS
13
ESTRÉS OXIDATIVO Y ANTIOXIDANTES EN
ESPERMATOGENESIS
14
CICLO ESTRAL
15
CICLO OVULATORIO Y MENSTRUAL

• Escudero F, Gonzales GF, Góñez C. Hormone profile
  during the menstrual cycle at high altitude.
  International Journal of Gynecology and
  Obstetrics 199655 49-58
• Gonzales GF, Villena A. Critical anthropometry
  for menarche. Journal of Pediatric and Adolescent
  Gynecology. 19969139-143

16
Menarquia y Menopausia

• Gonzales GF, Villena A. Body Mass Index and Age
  at menarche in Peruvian children living at high
  altitude and at sea level. Human Biology 1996
  68 265-275.
• Gonzales GF, Villena A, Ubilluz M. Age at
  menarche in Peruvian girls at sea level and at
  high altitude Effect of ethnic background and
  socioeconomic status. American Journal of Human
  Biology 19968 (4) 457-464
• Gonzales GF, Ortíz I. Age at menarche at sea
  level and at high altitude in Peruvian women from
  different ethnic composition. American Journal
  of Human Biol. 19946 627-640

17
Perimenopausia y Menopausia

• Gonzales GF, Villena A. Age at menopause in
  Central Andean Peruvian Women. MENOPAUSE, The
  Journal of the North American Menopause Society.
  1997432-38
• Gonzales GF, Villena A, De La Cruz D. Age at
  natural menopause among women in Lima City, Peru.
  International Journal of Gynecology and
  Obstetric. 199757 69-72.
• Gonzales GF. High serum follicle stimulating
  hormone (FSH) during perimenopause at high
  altitude. International Journal Gynecology and
  Obstetrics. 2000 68159-161.

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CONDUCTA SEXUAL EN ANIMALES DE EXPERIMENTACION

• Gonzales GF, Ortega JG, Salazar M. Effect of
  neonatal administration of an antidopaminergic
  drug (Metoclopramide) on sexual behavior of male
  rats. Archives of Andrology, 2000 45137-142.

19
Deseo sexual en varones

• Gonzales GF, Córdova A, Vega K, Chung A, Villena
  A, Góñez C, Castillo S. Effect of Lepidium
  meyenii (Maca) on sexual desire and its absent
  relationship with serum testosterone levels in
  adult healthy men. Andrologia 2002 34 367-372.

20
SITIOS DE IMPLANTACION Y SACOS EMBRIONARIOS

• Ruiz-Luna AC, Salazar S, Aspajo NJ, Rubio J,
  Gasco M, Gonzales GF. Lepidium meyenii (Maca)
  increases litter size in normal adult female
  mice. Reproductive Biology and Endocrinology
  2005 3(1)16

21
INFERTILIDAD Y PROCEDIMIENTO DE PREPARACION DE
ESPERMATOZOIDES

• Gonzales GF, Pella RE. Swim down A rapid and
  easy method to select motile spermatozoa.
  Archives of Andrology 19933029-34

22
ANALISIS DEL LIQUIDO SEMINAL

• Gonzales GF. The corrected seminal fructose test.
  Archives of Andrology 199433 17-22
• Gonzales GF, Villena A. Influence of low
  corrected seminal fructose levels on sperm
  chromatin stability in semen from men attending
  an infertility service. Fertility and Sterility
  199767 763-768.

23
ANALISIS DEL LIQUIDO SEMINAL

• Gonzales GF, Córdova A, Gonzales C, Chung A, Vega
  K, Villena A. Lepidium meyenii (Maca) increased
  semen parameters in adult men. Asian Journal of
  Andrology. 2001 3 301-303 .

24
BIOQUIMICA SEMINAL

• Gonzales GF. Response of the epididymis, seminal
  vesicles and prostate to the endogenous androgen
  stimulation in men attending an infertility
  service. Andrologia. 2002 34 308-316
• Gonzales GF. Function of seminal vesicles and
  their role on male fertility. Asian Journal of
  Andrology. 2001 3(4)251-8.

25

• Gonzales GF, Villena A. True Corrected seminal
  fructose level A better marker of the function
  of seminal vesicles in male infertility.
  International Journal of Andrology. 2001 24
  255-260
• Gonzales GF, Salirrosas A, Torres D, Sánchez A,
  Villena AE. Clomiphene citrate in the treatment
  of men with high sperm chromatin stability.
  Fertility and Sterility. 1998 69 1109-1115

26
PROSTATA

• Gonzales GF, Miranda S, Nieto J, Fernandez G,
  Yucra S, Rubio J, Yi P, Gasco M. Red Maca
  (Lepidium meyenii) reduced prostate size in rats.
  Reproductive Biology and Endocrinology. 2005
  3(1)5

27
Prostatic epithelial height in control rats, rats
treated with Red Maca alone, testosterone
enanthate (TE) alone or TERed Maca. A. Red Maca
was administered for 14 days. B. Red Maca was
administered for 42 days. aPlt0.05 respect to
vehicle bPlt0.05 respect to Red Maca cPlt0.05
respect to TE.
28
Prostatic luminal area in control rats, rats
treated with Red Maca alone, testosterone
enanthate (TE) alone or TERed Maca. B. Red Maca
was administered for 42 days. aPlt0.05 respect to
vehicle bPlt0.05 respect to Red Maca cPlt0.05
respect to TE.
29
Saturación de oxígeno

• Gonzales GF, Salirrosas A. Pulse oxygen
  saturation and neurologic assessment in human
  neonates after vaginal and cesarean delivery.
  International Journal of Gynecology and
  Obstetrics. 1998 6363-66
• Gonzales GF, Salirrosas A. Pulse oxygen
  saturation and neurologic assessment in human
  neonates after vaginal and cesarean delivery.
  International Journal of Gynecology and
  Obstetrics. 1998 6363-66
• Gonzales GF, Villena AE. Low pulse oxygen
  saturation in post-menopausal women at high
  altitude is related to high serum
  testosterone/estradiol ratio. International
  Journal of Gynecology and Obstetrics,
  200071147-154.

30
Mean pulse oxygen saturation in newborns at 40-41
weeks of gestational age at sea level and at high
altitude (4340 m). Data are mean ? SEM. F value
2146.97, Plt0.0001 (Two-way ANOVA test).Plt0.001
with respect to values at sea level.