Diapositiva 1

1
Grup de Sensors i Biosensors Departament de
Química Universitat Autònoma de Barcelona
Bellaterra Catalonia, Spain
Arben Merkoçi, PhD
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• OBJECTIVE
• REPRESENT OUR CANDIDATURE AS POSSIBLE PARTNER IN
  A PROJECT RELATED WITH OUR EXPERTISE
• 2. COORDINATE A STREP PROJECT

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SOME DATA ON GSB ?Creation of the group Year
1983 ?280 scientific publications ?300
presentations in international congresses ?4
patents on construction methods of all-solid
state potentiometric sensors and flow injection
systems. Some of these instruments are
commercially available (ADASA Barcelona) ?1
pending patent ?25 PhD thesis supervised ?More
than 60 projects with public or private financial
support ?Permanent research links with
Microelectronics National Centre, Institute of
Material Science and clinic and veterinarian
university hospitals staff 5 Permanent
professors 3 Senior scientists Director
Prof. Salvador Alegret
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• Research interest of GSB
• DESIGN OF DISPOSABLE SENSORS
• Signal transducing platforms for sensors based
  on enzymes, antibodies and DNA
• Potenciometric, amperometric, impedance and
  optical devices.
• Applications in clinics, environmental and
  industry.
• DEVELOPMENT OF AUTOMATIC ANALYSERS
• Design of automatic analytical and bioanalytical
  systems based on magnetical
• particles fluidics. Environmental applications.
• INTEGRATION OF NOVEL SMART MATERIALS IN SENSING
  SYSTEMS
• Composites
• Biocomposite
• Magnetic microparticles
• Nanoparticles

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• SPECIFIC TARGETED RESEARCH PROJECT (STREP)
• TENTATIVE TITLE
• DEVELOPMENT OF NOVEL USER-FRIENDLY SENSING
  SYSTEMS BASED ON QUANTUM DOTS (QD), CARBON
  NANOTUBES AND OTHER NANOPARTICLES (NP)
• OBJECTIVE / HYPOTHESES
• Synthesis and conjugation of QDs other NPs
  along with their integration in disposable as
  well as automatic sensing systems
• Show the ability of QDs along with other NPs as
  alternative to labelling using enzymes or other
  materials

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QUANTUM DOTS (QDs) AS BIOLOGICAL MOLECULE TRACERS
Monodisperse inorganic nanocrystalline
particles made from semiconducting materials,
typically 2-10nm in size from a single electron
to a collection of several thousands CONJUGATED
QDs NOW AVAILABLE (connections with proteins,
nucleic acids, or small molecules ) Use as bar
codes to create highly multiplexed assays
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• APPROACH
• Main concept

NP2
NP1
NP5
NP4
NP3
NP4
NP5
NP3
NP2
NP1
Immobilised DNA strands
II-Hybridisation with NP-modified DNA probes
I- Hybridisation with DNA analytes
Electrochemical detection
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APPROACH
Why electrochemical detection?

• HIGH SENSITIVITY
• INHERENT MINIATURIZATION
• COMPATIBILITY WITH MODERN MICROFABRICATION
  TECHNOLOGIES
• INDEPENDENT OF TURBIDITY AND OPTICAL PATHWAY
• INNOVATIVE ROUTES FOR INTERFACING DNA RECOGNITION
  AND SIGNAL TRANSDUCTION

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• APPROACH
• The proposed research will have the following
  main components
• Synthesis of NPs for sensor applications
• Modifications of NPs with biological molecules
• Physical and electrochemical characterization
• Application in sensing systems
• Applications by end-users

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• APPROACH
• Main components
• Synthesis of NPs for sensor applications
• Based on NPs with electrochemical properties.
• Requirement of the synthesised NC
• easy to be detected by any conventional
  electroanalytical or other method
• size distribution as narrow as possible so as to
  ensure enough reproducibility during the
  electrochemical assays where will be used as
  biological molecule labels
• colloidal NC, dispersed in a solvent, should be
  stabilized in a way that prevents agglomeration

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• APPROACH
• Main components
• 2. NPs modifications with biological molecules
• NP integration into/onto other particles
• Linking with antibodies
• Linking with DNA
• 3. Physical and electrochemical
  characterization
• - Optical
• - Electrochemical

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• APPROACH
• Main components
• 4. Application in sensing systems
• Optical
• Electrochemical
• 5. End-user demonstration
• - DNA
• - Immunosensor

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SCHEMATIC ROADMAP OF THE PROJECT
Optical applications
Electrochemical applications
NC of optimal compositions
Validation demonstration
Electrochemical sensors
Optical sensors
Production of prototypes and dissemination
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• EXPECTED RESULTS CONCLUSIONS
• Nanoparticles in general and QDs in especial, may
  be expected to be superior in several ways.
• Compared with existing labels they are more
  stable and cheaper
• They allow more flexibility, faster binding
  kinetics (similar to those in a homogeneous
  solution)
• High sensitivity and high-reaction speeds for
  many types of multiplexed assays, ranging from
  immunoassays to DNA analysis.
• Nanoparticles offer elegant ways for interfacing
  DNA recognition events or immunorecognition
  signal transduction processes and can bring novel
  capabilities to the hybridization or
  immunoreaction sensing. The creation of DNA or
  protein array chips can be envisioned

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• THANKS