Diapositiva 1

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PERFORMANCE IN ERNDIM SPECIAL ASSAYS FOR
CREATININE DISORDERS
Alberto Burlina Cas
Weykamp
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creatinine
Clinica Chimica Acta 361 (2005) 1 9
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ERNDIM Special Assay Schemes Analytes 2007
Urine 5-OH-Indolacetic acid Carnitine
free Creatine Creatinine Guanidinoacetate Homovani
llic acid Hydroxyproline Lactic
acid Mucopolysaccharides Orotic acid Pipecolic
acid Sialic acid Succinylacetone Uric acid
Serum 3-OH Butyric acid 7-Dehydrocholesterol Acet
o acetate C220 Behenic acid C240 Lignoceric
acid C260 Cerotic acid Carnitine
free Creatine Guanidin acetic acid Galactose Homoc
ysteine Lactic acid Phytanic acid Pipecolic
acid Pyruvic acid
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S-Guanidinoacetate (17)
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S-Creatine (19)
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U-Creatinine (70)
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U-Creatine (24)
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U-Guanidinoacetate (26)
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Methodology and Quality Assurance in IEM
Principles
Parameters for method validation(details ERNDIM
web-site, http//www. erndimqa.nl)

• Recovery
• Range
• Linearity? Limit of detection? Limit of
  quantitation

• Specificity
• Selectivity
• Precision? Repeatability? Reproducibility
• Accuracy

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Special assays serum 2007
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Special assays urine 2007
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PROPOSAL
Special Assays   a. Remove of hydroxyproline
  b. Low level mevalonate   c. Low levels
carnitine  d. Low levels 3-hydroxybutyrate,
urate and creatine