ctaTACTCATATATTGGTAtac

1
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2
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ctaTACTCATATATTGGTAtac tgtTACTCATTTATGGGTAagt actT
ACCTATATAGGGGTAatc tacTACCCCTATATGAGTAatg tggTACTC
ATATAGGGGTActg atgTACCCATATATGAGTAtca agcTACCTATAT
ATAGGTAgaa . . . TAC T A GTA
????????? ???????
???????????? ??????????????????
??A AT TGG TTC TA GAA
????????? ???? ctacattaagatcgcttcactaaaccaTAaTT
CTAcAGGGGTttattatgcggaaactctggaacgcg ??? ?????
13/16
3
????? ???????????? ??????
??????? ?????
??????? ?????
TACTCATATATTGGTA TACTCATTTATGGGTA TACCTATATAGGGGTA
TACCCCTATATGAGTA TACTCATATAGGGGTA TACCCATATATGAGT
A TACCTATATATAGGTA
??????? a c g t 1 -0.25
0.06 -0.25 0.44 2 0.38 -0.08 -0.31
0.00 3 0.17 0.33 -0.17 -0.33 4
-0.16 0.36 -0.32 0.13 5 0.07 0.10
-0.40 0.23 6 0.07 0.30 -0.37 0.00
????????? ???????
??? ??????? ?????????? ??? ?????? ???????
????????? Logo ??????????? ??????????? ???????
????????? ???? ctacattaagatcgcttcactaaaccaTAATT
CTACAGGGGTTtattatgcggaaactctggaacgcg ??? ?????
3.91
4
????????????? ?????? ? ???????? ?????????
????? ????????????? ????????????
????
????? 1
5
????????????? ?????? ? ???????? ?????????
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????? 1
6
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????? ????????????? ????????????
????
????? 1
7
???????? ??????????? ???? ????????
?????? 3 ??????????? ????????
?????? 1 ??????????????? ????????
?????? 2 ??????
8
???????????? ??????????? ????? ???????? ??
??????? Escherichia coli
?????? ??????????
????????? ??????????
?????????????
??????
?????????
9
????????? ????????? ????? ??????? ? Escherichia
coli

• FNR ????????? ??????????? ? ?????????
  ????????? ???????
• ArcA ????????? ????????? ??????????? ? ?????
  
• ?? ??????????? ?????????
• NarL ? NarP ????????? ?????????? ?
  ?????????? ???????
• ? ????????? ?????? ????? ???????????
  ???????

• ??? ??? ????? ????? ???? ??? ????????????,
• ??? ? ????????????

10
????????? ? ??????? ????? FNR
O2
O2
11
????????? ? ??????? ????? ArcA
O2
O2
12
????????? ? ??????? ?????? NarL ? NarP
NO3
NO3
13
????? ?? ?????? ? ?????? ? Escherichia coli
????????
??????
?????????
ctgTACCCATaaaaactaATGGGTAtca aTACCCATctgaaaaactacT
ACCCATg actgaaaaaTACCCATctaatccctatg . . . . . .
. . . .
actgaaaTACCCATaaATGGGTAtaatc
????
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???? ?????????? ????? FNR
??????????????? ??????
??????
Gerasimova A.V., Rodionov D.A., Mironov A.A,
Gelfand M.S. (2001) Computer analysis of
regulatory signals in bacterial genomes. Fnr
binding segments. Molecular Biology, 35,
1001-1009.
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???? ?????????? ????? ArcA
??????? ? ??????? ????????? SeSiMCMC
??????
1. Gerasimova A.V., Gelfand M.S., Makeev V.U.,
Mironov A.A., Favorov A.V. (2004) ArcA
regulator of gamma-proteobacteria identification
of the binding signal and description of
the regulon. Biophysics in press
2. http//favorov.hole.ru/gibbslfm/
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???????????????? ?????? ?????? NarL ? NarP
NarL
NarP
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???????????????? ?????? ?????? NarL ? NarP
NarL
NarP
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??? narP ?????? ??????????? ?????? ? ??????

• narQ
• napFDAGHBC
• nrfABCDEFG
• ccmABCDEF-dsbE-ccmH

• ????????? ?????
• ?????????????????? ??????-?????????
• ?????????????????? ??????-?????????
• ??????? ???? ? ??????????

???? ?????????? ????? NarP
?????????
19
?????-??????????????, ?????????? ?????? NarP
????????? Enterobacteriaceae
????????? Pasteurellaceae
????????? Vibrionaceae
20
?????????? ??????
????????????? ? ???????????? ???????
Escherichia coli
21
?????????? ??????
????????????? ? ???????????? ???????
Yersinia pestis, Yersinia entercolitica
22
?????????? ??????
????????????? ? ???????????? ???????
Haemophilus influenzae, Pasteurella multocida,
Actinobacillus actinomycetemcomitans
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?????????? ??????
????????????? ? ???????????? ???????
Haemophilus ducreyi, Vibrio fischeri, Vibrio
cholerae, Vibrio vulnificus, Vibrio
parahaemolyticus
24
?????????? ??????
NarP - ???????
1. ???????? ??? ????, ???????????? ? Escherichia
coli ??????? ???????? NarL NarP 2. ????
????????
25
?????????? ??????
????? ????? ?????????
Yersinia pestis, Haemophilus influenzae,
Actinobacillus actinomycetemcomitans, Haemophilus
ducreyi, Vibrio cholerae, Vibrio vulnificus,
Vibrioparahaemolyticus, Vibrio fischeri
FNR, ArcA, NarP
FNR, ArcA
Yersinia entercolitica
FNR
Pasteurella multocida
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?????????? ??????
????? ????? ?????????
????????????? ??????
Escherichia coli
Yersinia pestis Fnr ArcA Yersinia
entercolitica Fnr Pasteurella
multocida Fnr ArcA NarP Actinobacillus
actinomycetemcomitans ArcA NarP Haemophilus
influenzae Fnr ArcA Haemophilus
ducreyi Fnr ArcA NarP Vibrio vulnificus
ArcA Vibrio parahaemolyticus ArcA
Vibrio cholerae Fnr ArcA Vibrio
fischeri ArcA
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?????????? ??????
????? ????? ?????????
?????? ??????? ????????-??????????? ?????????
Yersinia pestis Fnr Yersinia
entercolitica Fnr ArcA Pasteurella
multocida Fnr ArcA Actinobacillus
actinomycetemcomitans Fnr NarP Haemophilus
influenzae Fnr NarP Haemophilus
ducreyi Fnr ArcA NarP Vibrio vulnificus
NarP Vibrio parahaemolyticus NarP
Vibrio cholerae NarP Vibrio
fischeri ArcA NarP
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??????

1. ??????? ????????? ??????????? ????????????
   ???????????? ??????, ????????? ?? ?????????, ??
   ?????????????? ??????? ????????????? ????????.
2. ??????????? ? ??????????? ???????? ?????????
   ?????????-??????????? ?????????.
3. ??????? ???????????? ??????? ??? ?????? ??????
   ?????????? ?????? ArcA ? NarP.
4. ??????? ????? ????????????? ????? ?????????????
   ?????????.
5. ?????????? ??? ???????????? ????????, ????????
   ????????? ???????? ??????????? ? ???? ????????.

29
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