Diapositiva 1

1
Table S1. Primer sequences used for the
amplification of barley genes in qRT-PCR
assays. Icy1 to Icy13 (cystatin genes) HvPap4,
HvPap6, HvPap10, HvPapa16 and HvPap17 (cathepsin
L-like protease genes) and Actin2 (actin gene).
Genes Primers
Actin2 forward 5-TCCACCGGAGAGGAAGTACAGT-3 reverse 5-AATGTGCTCAGAGATGCAAGGA-3
Icy1 forward 5-CGGCCGCCTAATGAAAAGA-3 reverse 5-CATATTACGCCAGTATCCAGCG-3
Icy2 forward 5-AAACTGTTGCTGGCACCATGT-3 reverse 5-GGATGTGCCTTATGCAATTGC-3
Icy3 forward 5-ATGGCACCCAAGATGCAC-3 reverse 5- TTCAGAACCTCATGTTCCGACC-3
Icy4 forward 5-TTGTTGAATGCGGCACGAT-3 reverse 5-GCAGCCAACAGTACCCTGAGTT-3
Icy5 forward 5-CTGCGTGTCTTTTTTGCGC-3 reverse 5-CCTGCCTCATATCGTACAGCCT-3
Icy6 forward 5-TGCCGGTTAAATTCATCTGCTC-3 reverse 5-CAGGAACTGAATGCCTGACGA-3
Icy7 forward 5-TTGGTGTCAAGGTTGGCATGT-3 reverse 5-CCAAATCGATCGCTTGGTTG-3
Icy8 forward 5-CTACGTCGCCGAGGACTACGAG-3 reverse 5-TCAAAGGGGCGAGCGGACGAG-3
Icy9 forward 5-CGTCTCCCAAATAATCGACAGG-3 reverse 5-CGAGAAAAAGAGTGCAACCAGG-3
Icy10Icy13 forward 5-AACCAGTTGTGGAGCACAACA-3 reverse 5-TGAACTCCAACACTGCCCAA-3
Icy11 forward 5-TATCAGCTCGTCATTGACGCC-3 reverse 5-TGAACTTTCGTGTCTTCGCG-3
Icy12 forward 5-TCTGTGTCATCGTTGTTGCGA-3 reverse 5-CCCCTGGATATGTTGGTCGTT-3
HvPap4 forward 5-CCTTGAGAGTCCTTGTTCCCGA-3 reverse 5-CCATGTTCGTCGTTTTAACCGA-3
HvPap6 forward 5-TGCAATTGACGGCAAGAAGA-3  reverse 5-TGGATCACCAGGTGATCATTTG-3
HvPap10 forward 5-TCGATCCATGTGCTTATCCGA-3 reverse 5-AACACACGCCTAATCCTTGGC-3
HvPap16 forward 5-CTGGATCGGTAAGAACTCGTGG-3 reverse 5-TGATGGAGGTGCCATCATATGA-3
HvPap17 forward 5-AGCTGCGTGTGCATTTATCATG-3 reverse 5-GCGGTGAAATATGCAACCCA-3
2
HvCPI-1
HvCPI-6
HvCPI-2
HvCPI-3
HvCPI-4
HvCPI-5
HvCPI-7
HvCPI-11
HvCPI-8
HvCPI-9
HvCPI-10
HvCPI-12
HvCPI-13
Fig. S1
3
Fig. S2
4
HvCPI-1
HvCPI-13
HvPap-10
HvPap-4
Fig.S3
5
Fig. S4