Nessun titolo diapositiva

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The Arabidopsis Italian Mafia

• Who we are?
• What we are doing?
• Who is going to pay?
• And..FIRB Arabidopsis
• an Italian Functional Genomics Program
• On Arabidopsis

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MORELLI G. GROUP. INRAN, ROME
Funding Ministry of Research grants
RUBERTI I. GROUP. CNR, ROME

• Functional Genomic analysis of Arabidopsis TFs
  REGIA
• Responsible scientists Dr. M. CARABELLI,
  Dr. G. SESSA

Funding EU FP5 Programme REGIA project EU FP5
Programme TF-stress project Ministry of
Research grants
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COSTANTINO GROUP. Dept. Gen. And Mol.Biol. Univ.
ROME

• Functional analysis of DOF proteins in A.t.
  Resp. scientist Dr. P.VITTORIOSO

• Functional Genomic analysis of Arabidopsis TFs
  REGIA
• Responsible scientist Dr. P.
  VITTORIOSO

• rolD and flower transition Responsible
  scientist Dr. M. TROVATO

Funding EU FP5 Programme REGIA
project Ministry of Research grants Institut
Pasteur -Fondazione Cenci Bolognetti grant
TONELLI GROUP. Dept. Gen. And Microbiol. Univ.
MILAN

• Large-scale Exon-trapping System Responsible
  Scientist Dr. M.GALBIATI
• (EXOTIC)

• Functional analysis of MYB NF-Y TFs in A.t.
  Resp. scientist Dr. K. PETRONI

Funding EU FP5 Programme REGIA project EU FP5
Programme EXOTIC project Ministry of Research
grants
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COLOMBO GROUP. Univ. MILAN

• Functional analysis of MADS proteins in A.t.

• Functional Genomic analysis of Arabidopsis TFs
  REGIA

Funding EU FP5 Programme REGIA
project Ministry of Research grants
ALPI GROUP. Univ. PISA PERATA P. (Uni MO e RE),
LORETI E. (CNR)

• Sugar sensing mechanisms and cross-talk with
  hormonal signaling

MARMIROLI GROUP. Dept. of Environ. Sciences Univ.
PARMA MARMIROLI N., VISIOLI G., GULLI M., MAESTRI
E.

• Isolation of mutants resistant to heavy metals
  from T-DNA collections

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SOAVE GROUP. Dept. of Biology, Univ. MILAN SOAVE
C., MORANDINI P, MURGIA I., TARANTINO D.

• Study of proteins that interact with H-ATPases
  proton pumps.
• Isolation of A.t. mutants altered in the
  sensitivity to photoinhibition, in the active
  oxygen scavenging processes, in the sensitivity
  to UV-B radiation, in the chirality of the
  primary roots

MIGLIACCIO GROUP. Inst. Plant Biochem.
Ecophysiol.,CNR.Monterotondo RM MIGLIACCIO F.,
PICONESE S., FAGGIANO M., ROSI C.

• Isolation of A.t. mutants affected in the
  gravitropic response,
• Isolation of A.t. mutants affected in auxin
  physiology
• Isolation of A.t. mutants affected in the basal
  process of circumnutation,
• Functional Analysis of the AtRHA1 gene

Funding European Space Agency (ESA) Italian
Space Agency (ASI)).
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FIRB Arabidopsis From Arabidopsis to Tomato a
scientific network and a technological platform
for the functional genomics of plant development
The proposal intends to exploit a functional
genomics approach to analyse selected regulatory
aspects of Arabidopsis and Tomato development
through the analysis of the function and
interactions of members of different families of
regulatory and structural genes.
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The proposal will focus on
- vegetative aerial development
- reproductive development
Transfer to Tomato of results and resources
already available to some of the UR allows to
consider two applied objectives on.
- plant architecture
- nutritional quality
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PARTICIPANTS
PAOLO COSTANTINO (COORDINATOR) Università "La
Sapienza", ROMA
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The genes analysed in the proposed research will
be
- Dof family (UR Costantino) involved in
auxin-dependent meristem formation, in the
control of seed germination and in the response
to gibberellins and stress - rol family (UR
Costantino) that influence meristem formation,
floral transition and sexual organ formation
- HD-Zip family (UR Ruberti) (UR Morelli)
involved in the regulation of primary and
secondary meristem activity and in developmental
processes as a response to the environment, such
as shade-avoidance response
- MYB family (UR Tonelli) involved in
morphogenesis, stress response and in the
biosynthesis of nutritionally relevant polymers -
NF-Y family (UR Tonelli) which interact with
several families of transcription factors crucial
in differentiation and development in eukaryotes
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- MADS (UR Colombo) involved in vegeative and
reproductive development -HMG and TAF (UR
Colombo) important factors in modulating
transcription
- PHY (UR Bowler) involved in the response to R
and FR light - Signalosome assembly (UR Bowler)
DET genes - CRY (UR Bowler, UR Benvenuto)
involved in the response to blue light
- Carotenoid biosynthesis (UR Benvenuto, UR
Cellini) important for photosynthetic activities
and nutritional quality
- PG, PGIP (UR Cervone) involved in the response
to pathogens and development
- 14.3.3 protein class (UR Aducci) involved in
cell cycle control and in several
signal-transduction pathways
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Expected scientific results

• Identification of genes involved in growth and
  development of the aerial part of the plant in
  Arabidopsis and Tomato
• Identification of genes involved in reproductive
  development in Arabidopsis and Tomato
• Identification of genes specifically expressed
  in the vegetative and floral apical region in
  Arabidopsis
• Identification of regulatory complexes through
  analysis of protein-protein interactions by means
  of
• Fluorescence Resonance Electronic Transfer
  (FRET) Surface Plasmon Resonance (SPR)
  2-hybrid FRET 2-hybrid in plant
• Identification of the function and interactions
  of members of different regulatory and structural
  gene families in vegetative and reproductive
  development

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Expected technological results
Development and utilisation of the following
technologies

• RNA interference (RNAi)
• Gain of function and negative dominant mutations
• Chemical-gene machine (TILLING)
• DNA microarray and macroarray for RNA profiling
  in Arabidopsis and Tomato
• Real-time PCR.

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Expected biotechnological results

• Modification of tomato plant architecture
• Modification of the nutritional quality and
  biofarming in tomato

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Expected infrastructural results

• Establishment of the first italian research
  network on plant functional genomics
• Training of scientific and technical personnel
  in functional genomics