Supplementary Fig. 1

1
Supplementary Fig. 1
Supplementary Figure 1 SDS PAGE analysis of
Os3BGlu6 purification. M. Bio-Rad low molecular
weight markers lane 1, crude protein extract of
Origami (DE3) cells after Os3BGlu6 expression
lane 2, fusion protein from initial IMAC
purification lane 3, Os3BGlu6 protein after TEV
protease digest lane 4, wash fractions after 2nd
IMAC, showing gt95 pure Os3BGlu6 protein.
Approximately 5 mg of protein was loaded in each
lane.