Supplementary Fig. 1

1
Supplementary Fig. 1
A
LN443
LN443X
B
C
LN443
LN443X
LN443X
LN443
U373
U373X1
U373
U373X1
D
E
LN443
LN443X
LN443
LN443X
U373
U373X1
U373
U373X1
2
Supplementary Fig. 2
A
LN443
LN443X
Paxillin
Merge
Actin
Paxillin
Actin
Merge
B
U373
U373X1
Paxillin
Paxillin
Actin
Actin
Merge
Merge
3
Supplementary Fig. 3
AEBP1 Number of samples in group
Up-Regulated 246 (71.7)
Down-Regulated 16 (4.7)
Intermediate 81 (23.6) Log-rank
p-value Up-Regulated vs. Intermediate 3.3E-8
Up-Regulated vs. Down-Regulated 8.0E-4
Down-Regulated vs. Intermediate 0.47
EREG Number of samples in group
Up-Regulated 6 (1.7)
Down-Regulated 15 (4.4)
Intermediate 322 (93.9) Log-rank
p-value Up-Regulated vs. Intermediate
0.31 Up-Regulated vs. Down-Regulated
0.55 Down-Regulated vs. Intermediate 0.74
MFAP5 Number of samples in group
Up-Regulated 32 (9.3)
Down-Regulated 6 (1.8)
Intermediate 305 (88.9) Log-rank
p-value Up-Regulated vs. Intermediate
0.054 Up-Regulated vs. Down-Regulated 0.27
Down-Regulated vs. Intermediate 0.54
4
Supplementary Fig. 4
GBM primary samples
G137 G144 G145
EREG - - -
100 ng/ml (20 µM)
p-EGFR
EGFR
p-ERK
ERK
5
Supplementary Fig. 5
A
B
EREG (membrane-bound)
U373X1
LN443X
si-EREG
si-EREG
LN443
U373
si-ctrl
si-ctrl
U373X1
C
U373
si-control
si-EREG
LN443X
LN443
si-control
si-EREG
6
Supplementary Fig. 6
A
U373
U373X1
U373X1-si-EREG
LN443
LN443X
LN443X-si-EREG
B
7
Supplementary Fig. 7
A
B
Verhaak et al., Cancer Cell 2010
Phillips et al., Cancer Cell 2006
U373X1 U373X2 LN443X
DLL3
NKX2.2
SOX2
ERBB3
OLIG2
FBXO3
GABRB2
SNCG
MBP
DNMT1
TOP1
ABL1
BOP1
FGFR3
PDGFA
EGFR
AKT2
NES
CASP1
CASP4
CASP5
CASP8
ILR4
CHI3L1
TRADD
TLR2
TLR4
RELB
U373X1 U373X2 LN443X
DLL3
SRRM2
SOX8
C20orf42
PIPPIN
dA201G10
GALNT13
NDRG2
NCAM1
RRP22
GABBR1
RRP22
GABBR1
SCG3
SNAP91
ATP6V1G2
KLRC3
RIL
PLA2G5
COL4A2
COL4A1
T1A-2
FAM20C
ANGPTL4
FLJ25348
SERPINE1
TAGLN
MYL9
LIF
FOSL2
YKL40
TIMP1
E2F7
RAMP
IQGAP3
HMMR
FKSG14
Mesenchymal Classical
Neural Proneuronal
Proliferative Mesenchymal
Proneuronal
8
Supplementary Fig. 8
A
LN443
U373
U373X1
LN443X
Sox2/actin
Sox2
B
LN443X
U373X1
LN443
U373
Sox2