Diapositiva 1

1
A
B
Supplementary on line FIG. S4. Western (A) and
Northern (B) analysis of leaf areas
agroinfiltrated with a combination of each of the
three constructs expressing the three OuMV
genomic RNAs (identified at the top of each lane)
and processed 5 days post agroinfiltration. For
the western analysis the top panel displays
reaction with antibodies for the movement protein
(MP), the middle panel displays reaction with
antibodies for the coat protein (CP) and the
bottom panel displays the total protein loadings
on an SDS-PAGE gel stained with the Coomassie
dye. The arrows indicate the position of the MP
and CP bands in the Western blot. In the
Northern analysis, from the top panel, minus
strand and plus strand probes for RNA 1, minus
strand and plus strand probes for RNA2 and minus
strand and plus strand probes for RNA3 were
hybridized with replicas of the same blot. The
bottom panel shows rRNA stained with methylene
blue on the membrane. MP and CP bands in the
Western analysis. The numbers expressing the size
of the marker bands are in kDa and kb
respectively for western and Northern blots.