Biobrick PowerPoint PPT Presentations

Biobrick Jiale Wang SUSTC Thank you ! Thank you ! Definition What is Biobrick ? BioBrick standard biological parts are DNA sequences of defined ...

BioBricks Part Types Chassis organism (E. coli, yeast, mammalian) Vectors plasmids, strains Parts - Proteins, RBS Devices Inverters, reporters Chassis ...

Idempotent Vector Design for Standard Assembly of Biobricks, Tom Knight 2001 ... Solar-Powered Sea Slugs. Rumpho et al. Plant Physiol 2000 ...

Terminator. Reporter. Coding. Region. Reporter. BioSketch ... Schedule - Week 3. P434 QPI857. BioBrick Assembly. Plambda-QPILac-GFP. Plac-QPIlambda-GFP ...

Beta: single stranded DNA binding protein, mediate recombination ... High Efficiency: 1/10. Goal: Construction of more efficient recombination system through ...

... 3 to the above left-end construct once again by PCR ligation as described above. ... Ligate the new BioBrick part into the original BioBrick plasmid ...

... Restriction Enzyme Digest, Ligation Reaction, & Gibson Assembly Activity: Make Your Own BioBrick (Exploring the Parts Registry) Follow-up Reading: ...

Further plans: readjust part to BioBrick standard. Combine part BBa_F1610 and BBa_I0500 (pBAD promotor) Possibly: clone CFP cassette behind this new sender part ...

Transform E. coli with Kai Biobricks to reconstitute KaiC phosphorylation cycle ... Blue Heron. DNA 2.0. Quote: $1.10/bp, 10-15 business days ...

Create KaiA, KaiB, and KaiC biobricks. ... Lanes 1, 6, 11: 1kb Lane 2 and 4: expected 210bp segment. Lane 3 and 5: expected 80bp segment ...

Bioware Lab Work Bootcamp! Clone two Biobricks from the parts registry and combine them in a plasmid. Projects Tom and Amanda constitutive pTet promoter and ...

... to reconstitute KaiC phosphorylation cycle with Biobrick'd luciferase reporter. ... Using PCR to create and extract the correct constructs. ...

Dezvoltarea limbajului Dezvoltarea social Apari ia elitelor (gen Arhimede) mbun t irea tehnologiilor de supravie uire i comunicare Celulele stem Biobricks ...

Title: Slide 1 Last modified by: Malcolm Campbell Document presentation format: Custom Other titles: Gill Sans ProN W3 Arial MS P ...

yo. sup. Test constructs. luxR. OHHL. BBa_I13263. BBa_T9002. Growth data for ... Construction of GFPmek for PDZ binding. Preparing sLppOmpA for future assembly ...

Cross reactivity between 3OC12/LuxR. Time Delayed Growth System. Varied temperature, ampicillin concentration, and agar concentration ...

Cell Surface Targeting

1. Addressable Bacterial Conjugation. UC Berkeley iGEM 2005. Michael Chen. Vlad Goldenberg. Stephen Handley. Melissa Li. Jonathan Sternberg. Jay Su. Eddie Wang ...

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Title: NASLOV (potrebno ga je e izbrati predlogi za enkrat: Unbeateable Cell SuperCell Changing the Path of Life Modification of TLR signaling ...

Michigan iGEM Team 07. RoSBM. Challenges. Modeling takes time, expertize ... Data Connectivity. Parts. Models. Exp.Data. Sim.Data. Parts. Parts. Exp.Data ...

Three proteins-- KaiA, KaiB, and KaiC interact to produce ... Prof. Susan Golden at TAMU for helping us with Western blot antibodies and general advice ...

To establish specific cell-to-cell communication between two populations of bacteria ... Dr. Laura Frost: F-Plasmid. Philip Silverman: pox38 F-Plasmid ...

Engineered Human Cells: ... Coagulation cascade Complement cascade Vessel injury Release of prostaglandins Eventually leads to multiple organ dysfunction syndrome ...

Anthrax relies on Protective Antigen (PA) to function (allows ... UPI0000027636. Mus musculus version of Anthrax Toxin Receptor. Remodel MIDAS region ...

... will measure oscillation by doing Western blots of our ... The problem: Will our E. coli preserve current cycle phase between mothers and daughters? ...

pQE-30 Colicin Cloning: Strategy. Clone of colicin genes in His-tag Vector pQE-30 ... Combination of existing receiver part with colicin gene cassettes in pSB1A3 ...

Prodigiosin Production in E. Coli Brian Hovey and Stephanie Vondrak What is Prodigiosin? A secondary metabolite of various strains of Serratia, and other Gram ...

Problem with implementation in later generations of the ... Rhythm has been shown to be driven by the interaction of three ... Create KaiA, KaiB, and ...

a new and rapidly emerging discipline that aims at the (re-)design and ... Fabricate materials and structures. Produce energy. Provide food. Process information ...

Cyanobacteria oscillation is robust and temperature-independent (within living tolerances). The oscillation period can be adjusted from 14-60hours by point ...

To offer a new way to target substrates to cells. ... To provide a foundation for engineered catalysts. Adaptamer testing. Bead surface. adaptamer ...

Express different photosynthetic pigments in cyanobacteria ... http://www.mbari.org/staff/conn/botany/reds/daisy/phycocyanobilin.gif ...

- Aptamers (Steph) - Bacterial Surface Display / Fusion Prot. (Perry) - Target Bact to certain tissues (actuator or product) - Targeting Microbial Factories

iGEM Principle of parsimony occam s razor What is iGEM? iGEM stands for iNternational Genetically Engineered Machines An undergraduate bioengineering ...

iGEM 2005

PCC7942 is the most common strain we've encountered in the literature on ... Susan Golden at UT Austin. Alexander van Oodenaarden at MIT. Jeffrey Chabot ...

Advisors: Professors Adam Arkin and Jay Keasling. GSIs: Chris Anderson and John Dueber ... Samantha Liang. Bryan Hernandez. Kaitlin A. Davis. Daniel Kluesing ...

Cell Surface Targeting contd

Sequencing for QuikChange-mutagenized single-chain dimer streptavidin showed ... Received new streptavidin clone (pTSA13) from Dr. Takeshi Sano ...

Cyanide Biosensor. Southern Utah University. Kurt Whittemore, Ray Uchida, Greg ... Pterin-Dependent Oxygenolytic Cleavage to Ammonia and Formate in Pseudomonas ...

iGEM Bioware Week 9 Arsenic Bioremediation Got ArsR into plasmid backbone and began site directed mutagenesis. using NEB competent cells significantly improved ...

Alain Viel, George Church, Pam Silver, William Shih, Radhika Nagpal, and Jagesh Shah. Prof. Susan Golden at TAMU for providing Western blot antibodies and advice ...

Promoters, Ribosome Binding Sites, Coding Sequences, terminators, Plasmids ... Taming Nature. Most parts are derived from natural systems. Building Devices ...

To create a fusion protein in E. coli expressing streptavidin on the surface ... functional monomers, Kd = 1.7x10-6 M. Qureshi, 2002: T90A, D128A, Kd = 1.3x10-8 ...

sigA strong hixL sigA weak hixR hin invertase CinR sigA LuxR LasR rcsA ter---GFP--RBS rcsA-RBS-SigA-LuxR-hixL- SigA- RBS- Hin-terR-terL-hinR-LasR-SigA-RBS-CinR-RBS ...

The first bacteria constitutively produces a diffusible quorum molecule which ... Fabricate master for PDMS mold. Test additional track designs ...

Can simple biological systems be built from standard, ... International Genetically Engineered Machine Competition ... powder, Ampicillin salt, ...

Buenos Dias! Meeting time for next week? Undergrad Research Symposium Reminder meeting with professors Friday the 16th from 5-6:30. Movie Night on Thursday

Genetic selection carried out for millenia (domestication of animals) Mendelian selection rationalized' process. Recombinant DNA. Engineering Goal: ...

The burnt pancake problem can be modeled as DNA Design of controlled flipping In Salmonella, Hin flips a ~1000 bp element in the flagellin operon.

Current field technology: the ... Arsenic-sensing bacteria with a pH output. Very sensitive. pH requires an indicator, litmus paper or a simple electrode ...

[1] Elowitz, M. and Leibler, S., A synthetic oscillatory network of ... team of 8 students won the Grand Prize. ... at least, iGEM prizes don't carry a ...

pH is easy, practical, and cheap to measure. Signal conversion: A B C where C is easy to detect ... Medical application. Altering Signaling Pathway ...

Background Project Goal Cyanobacteria, also known as blue-green algae, are a phylum of ancient photosynthetic bacteria. They are the simplest organisms known to ...

Photolithography. Went through microprinting process for mammalian cells in Kit's lab. ... Week 6 (7/11): Test cell signaling density; photolithography done ...