Adaptamers

1
Adaptamers
Target A
Target B
Adaptamer
2
Why develop adaptamers?

• To offer a new way to target substrates to cells
  (adaptamer linking cell surface and substrate).
• To create a tool for studying cell-cell
  interactions (adaptamer linking two cell
  surfaces).
• To provide a foundation for engineered catalysts
  (adaptamers linking 2 proteins).

3
Thrombin-binding domain
Streptavidin-binding domain
Thrombin
Linking region
Streptavidin
4
Adaptamer testing
streptavidin
adaptamer
thrombin
anti-thrombin antibody
Bead surface
5
Adaptamer functionality
Adaptamer A35
Adaptamer A15
adaptamer
Control no thrombin
streptavidin
Control beads lack streptavidin
thrombin
Bead surface
6
Adaptamer quenching
Displacement strand

Results
Displacement strand added

No displacement strand added
Background fluorescence