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ANTIMICROBIAL EFFECTS OF AQUEOUS AND GEL
CHLORHEXIDINE
C.C.P.GUIMARÃES1,2, S. CAI3, J.L.LAGE-MARQUES2 End
odontics - University Santo Amaro1 University of
São Paulo2 ICB-USP3 - Brazil
3325
clauvinajp_at_uol.com.br
INTRODUCTION
When growth occurred it was checked in every
instance for purity of E. Faecalis
Blocks were mounted in supports
Antimicrobial dressings have been used as
intracanal medications for many years although
the ideal formula has not been established.
Besides some microorganisms are more resistant
than others to root canal therapy. The aim of the
present study was to evaluate if different
vehicles enhance the antimicrobial efficacy of
chlorhexidine against Enterococcus faecalis.
Dentin blocks were sterilized (for 15 min at
121ºC )
They were infected with a standard suspension of
E. faecalis ATCC 29212
GROUP I - 2 aqueous chlorhexidine (n3)
MATERIALS AND METHODS
UFC of E. faecalis in TSA plates
Smear of E. faecalis in Gram stain
Blocks were divided into groups for application
of medications
GROUP II - 2 chlorhexidine with natrosol gel as
vehicle (n3)
Bovine incisors prepared in discs
All procedures were repeated six times
GROUP III - control (saline) (n2)
RESULTS
Canals were widened with an ISO 23 round bur
Incubation for 24 hr at 37 ºC
Group I
36
36
36
36
36
36
Group II
Scanning electron microscopic view from smeared
specimen (bovine dentin) after the round bur use
Dentin chips were taken with sterile round burs
of crescent sizes in tubes with TSB
Group III
24
24
24
24
24
24
Total tubes
Number of tubes
2
2
3
5
3
4
ISO
25
27
29
25
27
29
25
27
29
Results were analyzed using the chi-square test.
The smear layer was removed by immersion in 17
EDTA-T solution in a ultrasonic bath for 5 min
followed by 0,5 NaOCl for 5 min
CONCLUSION
Incubation for 24 hr at 37 ºC
Both medications were equally effective against
E. faecalis with no statistically significant
differences between them.
Scanning electron microscopic view from a
specimen (bovine dentin) after the ultrasonic bath
Afterwards, two new tubes were inoculated with
200 ?L of each tube (A, B and C)
Supported by University of Santo Amaro (UNISA)