Presentaci

1
(a)
LBb1
T-DNA
SALK_103855 (des1-1)
GW-CSL-F
DES1
GW-CSL-R
RATM13-2715-1_G (des1-2)
Ds-trans
DS3-4
(b)
DES1

UBQ 10
Wt des1-1
(c)
Figure S4. A, Intron-exon organization of the
DES1 gene (At5g28030) in the SALK__103855 and
RATM13-2715-1_G insertion mutants, named des1-1
and des1-2, respectively. LBb1, DS3-4, F and R
show the locations and directions of primers used
in the screening for mutant plants. The gene
structure and T-DNA region are not drawn to
scale. B, RT-PCR analysis of the mutant plants.
RNA samples were prepared from the leaves of
two-week-old plants, and primers specific for
DES1 and constitutive UBQ10 transcripts were
used. The des1-1 and des1-2 mutants are null
mutants. C, Southern blot analysis of the des1-1
mutant plant. Genomic DNA prepared from the
leaves was digested with XbaI and hybridized with
a T-DNA probe. The des1-1 mutant plants contain a
unique T-DNA insertion.