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MIKROORGANISME

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Title: MIKROORGANISME


1
MIKROORGANISME BAHAN ORGANIK TANAH
AGROTEKNOLOGI
2
(No Transcript)
3
In 1 teaspoon of soil there are
Bacteria 100 million to 1 billion
Fungi 6-9 ft fungal strands put end to end
Protozoa Several thousand flagellates amoeba One to several hundred ciliates
Nematodes 10 to 20 bacterial feeders and a few fungal feeders
Arthropods Up to 100
Earthworms 5 or more
Travis Gugino - PSU
4
Soil Organisms
  • Significance/Fungsinya
  • 1. Decomposition of plant and animal residues
  • 2. Release of nutrients and inorganic elements
    which feed plants and cause mineral weathering
  • 3. Synthesis of new organic compounds
  • 4. Humus formation to increase cation exchange
    and structure
  • 5. Nitrogen fixation

5
Soil Organisms Cont.
  • Kind
  • 1. Plant
  • 2. Animal
  • Plants ( bacteria, actinomycetes, fungi, algae)
  • 1. Bacteria
  • 1. Very small single celled organisms
  • 2. Multiply by elongation and dividing into 2
    parts
  • 3. Millions or even billions per gram of soil

6
I. Classification of Bacteria (Heterotropic)
  • A. Heterotropic obtain their carbon and energy
    from various organic compounds
  • 1. Nitrogen fixing derive their nitrogen in
    gaseous form from the atmosphere or they can
    obtain their nitrogen from Ammonia or Nitrates
  • a. Non Symbiotic - those that are free living
  • 1. Anaerobic organisms - those not needing
    free oxygen for respiration

Dont need Host Plant
7
  • Clostridium
  • More common in forest soils
  • Optimum Reaction pH 6.9 - 7.3
  • no fixation below pH 5.0
  • 2. Aerobic- need free oxygen
  • Azotobacter
  • More common in agriculture soils
  • Very sensitive to acidity
  • pH below 5.5 to 6.0 no fixation

8
b. Symbiotic - live on host plant to mutual
advantage Rhizobium - Find on nudules -
Nitrogen from the air c. Aerobic Bacteria-
requiring combined Nitrogen Bacillus
mycoides - Cause denitrification d.
Anaerobic Bacteria - which require combined
Nitrogen - Nitrates ---------- Nitrites,
ammonia - Sulfates ---------- Sulfites,
sulfides
9
Classification of Bacteria (Autotrophic)
  • B. Autotrophic- derive their carbon primarily
  • from CO2 of the atmosphere and their
  • energy from the oxidation of inorganic
  • compound or simple compounds of
  • carbon

10
Cont.
  • 1. Bacteria using nitrogen compounds as an
    energy source
  • a. Nitrosomes
  • - oxidize ammonium Nitrite
  • - NH4 NO2-

11
Cont.
  • b. Nitrobacter
  • - Oxidize Nitrite to Nitrate
  • - NO2- NO3-
  • - little or no activity below a pH of 6.0
  • - little or no activity below a temp of 65oF
  • 2. Bacteria using sulfur or sulfur compounds as
    energy sources.
  • a. Thiobacillus
  • S O2 H2O H2SO4

12
SOIL MICROORGANISM
Bacteria
UBC EM facility
Pseudomonas
Arthrobacter
CIMC
Bacillus
Travis Gugino - PSU
13
II. Actinomycetes
  • Unicellular micro-organisms
  • Transitional between the bacteria and fungi
  • Effect of soil pH
  • a. Sensitive to acid soil- no activity below pH
    5.0, optimum activity between 6.0 - 7.5
  • Heterotropic Feeders- breaks down organic matter
    and humus liberating nutrients, especially
    nitrogen
  • form NH3

14
Actinomycetes
Paul R. August
Streptomyces
Travis Gugino - PSU
15
Nitrogen Cycle
16
III. Fungi
  • Heterotropic organisms
  • Fungi will tolerate a wide pH range
  • abundant in acid soils where bacteria and
    actinomycetes offer only limited competition
  • Activities of Fungi
  • Decompose organic residues

17
Fungi Cont.
  • Micorrhiza- fungus roots
  • more common in forest soils
  • fungal roots form a mat around the absorbing root
    while others penetrate the root cells
  • Symbiotic relationship- help with nutrient
    absorption by increasing absorptive surface
  • Pine seedlings will not grow well without them

18
Microorganisms
Fungi
Aspergillus
Trichoderma
K.J. Kwon-Chung
PSU Em facility
D.C. Straney
Fusarium
Travis Gugino - PSU
19
Mycorrhizae
20
IV. Algae
  • minute plants which are photosynthetic
  • found in surface soils but in low amounts
  • Blue green types are important in wet soil
  • fix nitrogen in rice paddies
  • give off oxygen to the water

21
Soil Macro Animals
  • Include- rodents, insects, millipedes,
    centipedes, earthworms
  • Earthworm- most important macro animals
  • may pass as much as 15 tons of dry earth per acre
    through their bodies
  • Micro-organisms and the nitrogen cycle
  • Organic matter of mineral soils
  • Source- remains of plants and animals

22
Nematodes
23
Many beneficial effects from activities of
microorganisms
  • Microorganisms produce
  • Plant growth hormones
  • Stimulate plant growth hormones
  • Compete with disease organisms

24
  • SOIL ORGANIC MATTER
  • (BAHAN ORGANIK TANAH)

25
Soil ComponentsThe 4 parts of soil
26
Soil Organic Matter
  • SOM consists of a broad spectrum of chemical
    classes, including amino acids, lignin,
    polysaccharides, proteins, cutins, chitins,
    melanins, suberins, and paraffinic
    macromolecules, as well as organic chemicals
    produced by humans.

27
SOIL ORGANIC MATTER
  • Soil Organic matter
    encompasses all organic components of a soil
  • Fresh residues
  • Decomposing organic matter
  • Stable organic matter
  • Living organisms

28
  • Composition
  • of green tissue 75 is water
  • of dry matter in mature plants
  • Sugars and starches 1-5
  • Carbohydrates Hemicellulose 10-28
  • Cellulose 20-50
  • Fats, waxes, tannins, etc 1-8
  • Lignin's 10-30
  • Proteins Simples water soluble 1-15
  • and crude protein

29
Changes of Organic Compounds in the Soil
  • I. Compounds Characteristic of Fresh Plant
    Tissue
  • Decompose with difficulty Decompose easily
  • Lignin Cellulose
  • Fats Starches
  • Oils Sugars
  • Resin Proteins

30
  • II. Complex Intermediate Products of Decay
  • Resistant compounds Decomposition compounds
  • Resigns Amino acids
  • Waxes Amides
  • Oils and Fats Alcohols
  • Lignin Aldehydes

III. Products of Soil-Decomposition
Processes Resistant complex Simple end
products Humus- a colloidal complex carbon
dioxide and water nitrates,
sulfates phosphates, calcium
compounds
31
Rate of Decomposition
  • - Burning process - oxidation
  • 1. Sugars - Starches - simple proteins Rapid
  • 2. Crude proteins
  • 3. Hemicellulose
  • 4. Cellulose
  • 5. Lignins, fats, waxes Very Slow

32
The Carbon Cycle
- cycle of life energy cycle
CO2
Animal
To Atm.
Farm Manure
Green Manure Crop Residue
Soil Reactions
CO3 , HCO3
Microbial Activity
Carbon Dioxide
Drainage losses CO2 Carbonates
Bicarbonates of Ca, Mg, K, Etc.
33
Humus
  • Humus is a mixture of complex compounds and is
    not a single material. These compounds are
    either (a) resistant materials (b) compounds
    synthesized within microbial tissue.
  • Definition- Humus is a complex and rather
    resistant mixture of brown or dark brown
    amorphous and colloidal substances that have been
    modified from the original tissue or have been
    synthesized by various soil organisms

34
Humus Properties
  • 1. highly colloidal
  • 2. amorphous (not crystalline)
  • 3. cation exchange Cap. 150-300 me/100 gms.
  • Clay 8-100 me/100 gms.
  • 4. absorption of water from sat. atmosphere
    80-90 clay 15-20

35
Carbon Nitrogen Ratio
  • Carbon Nitrogen ratio fairly constant in soils
  • in cultivated soils 10 or 121 is a common ratio
  • in plant material Legumes 201
  • Straw 901
  • Sawdust 1501
  • Thus it can be seen that organic matter contains
    large amounts of carbon and comparatively small
    amounts of nitrogen.

36
Influence of Soil Organic Matter on Soil
Properties
  • 1. Soil Color - brown to black
  • 2. Influence on physical properties
  • - granulation encouraged
  • - plasticity cohesion reduced
  • - H2O holding capacity increased

37
  • 3. High cation adsorption
  • - 2 x 20 x clay
  • - 30 - 90 adsorbing power of mineral
  • soils
  • 4. Supply and availability of nutrients
  • - N, P and S held in organic forms
  • - Manure (10 - 5 - 10)/ ton (5 - 1 - 5)
    N P K Available

38
QUANTITY IN SOILS
  • SOM in mineral soil up to 18 by weight
  • Typically 0.5 to 5.
  • SOM gt 18 in organic soils
  • SOM about 50 C
  • SOM (OC)(factor) where the factor 1.8 to 2.0
  • For most estimations 2 is a good round number
  • Older books use a factor 1.724

39
How is SOM Measured?
  • SOM is usually measured in the laboratory as
    organic carbon,
  • Soil organic matter is estimated to contain 58
    organic carbon (varies from 40 to 58) with the
    rest of the SOM comprising of other elements (eg,
    5 N, 0.5 P and 0.5 S).
  • A conversion to SOM from a given organic carbon
    analysis requires that the organic carbon content
    be multiplied by a factor of 1.72 (1.00/0.58).
  • Thus, 2 SOM is about 1.2 organic carbon.

Testing for Soil Organic Carbon
40
Walkley Black
  • Oxidize SOM to CO2 using acid dichromate
  • Assume C is in the zero oxidation state as in
    carbohydrates (CH2O).
  • 3CH2O 16H 2Cr2(VI)O72- --gt 4Cr3 3CO2
    11H2O
  • Titrate excess Cr2(VI)O72- with Fe2
  • Not used much any more

41
Components of SOM
42
KESIMPULAN
  • Roles of Soil organisms Decomposition of plant
    and animal residues, Release of nutrients and
    inorganic elements, synthesis of new organic
    compounds, Humus formation, Nitrogen fixation
  • Soil organic matter all living organisms
    (microorganisms, earthworms, etc), fresh residues
    (old plant roots, crop residues, recently added
    manures), decomposing organic matter and stable
    organic matter (humus).
  • Influence of Soil Organic Matter on Soil
    Properties Soil Color - brown to black,
    Influence on physical properties (granulation ,
    plasticity , H2O holding capacity ), High
    cation adsorption, Supply and availability of
    nutrients.
  • SOM is usually measured in the laboratory as
    organic carbon, A conversion to SOM from a given
    organic carbon analysis requires that the organic
    carbon content be multiplied by a factor of 1.72
    (1.00/0.58).
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